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cloned into cho expression vector pcdna3.4  (Thermo Fisher)


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    Thermo Fisher cloned into cho expression vector pcdna3.4
    Cloned Into Cho Expression Vector Pcdna3.4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3%2E4+expression+vector/pmc11680381-129-19-23
    Average 90 stars, based on 1 article reviews
    cloned into cho expression vector pcdna3.4 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: A novel monoclonal antibody against human thymic stromal lymphopoietin for the treatment of TSLP-mediated diseases
    Article Snippet: Gene fragments encoding antibody heavy chain (HC) and light chain (LC) were synthesized by Genewiz (South Plainfield, NJ) or IDT (Coralville, IA) and cloned into pcDNA3.4 expression vector (ThermoFisher Scientific, San Jose, CA).

    Article Title: The Effects of Aβ 1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2
    Article Snippet: The cDNA encoding the antibody sequence was cloned into a pcDNA3.4 expression vector (Thermo Fisher Scientific, Waltham, MA, USA), and NP106 was expressed by an Expi-CHO cell system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol, followed by purification using protein G (GE Healthcare Chicago, IL, USA).

    Article Title: Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
    Article Snippet: The variable regions coding sequences were cloned into a pcDNA3.4 (Thermo Fisher) expression vector modified to contain coding sequences of a mouse IgKVIII signal sequence and the appropriate constant regions.

    Article Title: Immunogenicity of a spike protein subunit-based COVID-19 vaccine with broad protection against various SARS-CoV-2 variants in animal studies.
    Article Snippet: The DNA was then ligated into pcDNA3.4 expression vector (Thermo Fisher Scientific) using NEBuilder DNA Assembly kit (New England Biolabs).

    Article Title: Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model.
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific) with a polyhistidine tag at the N-terminus.

    Article Title: Immune evasion and chronological decrease in titer of neutralizing antibody against SARS-CoV-2 and its variants of concerns in COVID-19 patients
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a polyhistidine tag at the N-terminus.

    Article Title: Incomplete humoral response including neutralizing antibodies in asymptomatic to mild COVID-19 patients in Japan
    Article Snippet: The extracellular domain of ACE2 (NCBI, NM_021804.3, 1–708 AA) was cloned from cDNA of human PBMCs into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a streptavidin-binding peptide (SBP) tag or FLAG tag at the C-terminus.

    Clone Assay:

    Article Title: A novel monoclonal antibody against human thymic stromal lymphopoietin for the treatment of TSLP-mediated diseases
    Article Snippet: Gene fragments encoding antibody heavy chain (HC) and light chain (LC) were synthesized by Genewiz (South Plainfield, NJ) or IDT (Coralville, IA) and cloned into pcDNA3.4 expression vector (ThermoFisher Scientific, San Jose, CA).

    Article Title: The Effects of Aβ 1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2
    Article Snippet: The cDNA encoding the antibody sequence was cloned into a pcDNA3.4 expression vector (Thermo Fisher Scientific, Waltham, MA, USA), and NP106 was expressed by an Expi-CHO cell system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol, followed by purification using protein G (GE Healthcare Chicago, IL, USA).

    Article Title: Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
    Article Snippet: The variable regions coding sequences were cloned into a pcDNA3.4 (Thermo Fisher) expression vector modified to contain coding sequences of a mouse IgKVIII signal sequence and the appropriate constant regions.

    Article Title: Immunogenicity of a spike protein subunit-based COVID-19 vaccine with broad protection against various SARS-CoV-2 variants in animal studies.
    Article Snippet: The DNA was then ligated into pcDNA3.4 expression vector (Thermo Fisher Scientific) using NEBuilder DNA Assembly kit (New England Biolabs).

    Article Title: Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model.
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific) with a polyhistidine tag at the N-terminus.

    Article Title: Immune evasion and chronological decrease in titer of neutralizing antibody against SARS-CoV-2 and its variants of concerns in COVID-19 patients
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a polyhistidine tag at the N-terminus.

    Article Title: Incomplete humoral response including neutralizing antibodies in asymptomatic to mild COVID-19 patients in Japan
    Article Snippet: The extracellular domain of ACE2 (NCBI, NM_021804.3, 1–708 AA) was cloned from cDNA of human PBMCs into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a streptavidin-binding peptide (SBP) tag or FLAG tag at the C-terminus.

    Expressing:

    Article Title: A novel monoclonal antibody against human thymic stromal lymphopoietin for the treatment of TSLP-mediated diseases
    Article Snippet: Gene fragments encoding antibody heavy chain (HC) and light chain (LC) were synthesized by Genewiz (South Plainfield, NJ) or IDT (Coralville, IA) and cloned into pcDNA3.4 expression vector (ThermoFisher Scientific, San Jose, CA).

    Article Title: The Effects of Aβ 1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2
    Article Snippet: The cDNA encoding the antibody sequence was cloned into a pcDNA3.4 expression vector (Thermo Fisher Scientific, Waltham, MA, USA), and NP106 was expressed by an Expi-CHO cell system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol, followed by purification using protein G (GE Healthcare Chicago, IL, USA).

    Article Title: Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
    Article Snippet: The variable regions coding sequences were cloned into a pcDNA3.4 (Thermo Fisher) expression vector modified to contain coding sequences of a mouse IgKVIII signal sequence and the appropriate constant regions.

    Article Title: Immunogenicity of a spike protein subunit-based COVID-19 vaccine with broad protection against various SARS-CoV-2 variants in animal studies.
    Article Snippet: The DNA was then ligated into pcDNA3.4 expression vector (Thermo Fisher Scientific) using NEBuilder DNA Assembly kit (New England Biolabs).

    Article Title: Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model.
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific) with a polyhistidine tag at the N-terminus.

    Article Title: Immune evasion and chronological decrease in titer of neutralizing antibody against SARS-CoV-2 and its variants of concerns in COVID-19 patients
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a polyhistidine tag at the N-terminus.

    Article Title: Incomplete humoral response including neutralizing antibodies in asymptomatic to mild COVID-19 patients in Japan
    Article Snippet: The extracellular domain of ACE2 (NCBI, NM_021804.3, 1–708 AA) was cloned from cDNA of human PBMCs into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a streptavidin-binding peptide (SBP) tag or FLAG tag at the C-terminus.

    Plasmid Preparation:

    Article Title: A novel monoclonal antibody against human thymic stromal lymphopoietin for the treatment of TSLP-mediated diseases
    Article Snippet: Gene fragments encoding antibody heavy chain (HC) and light chain (LC) were synthesized by Genewiz (South Plainfield, NJ) or IDT (Coralville, IA) and cloned into pcDNA3.4 expression vector (ThermoFisher Scientific, San Jose, CA).

    Article Title: The Effects of Aβ 1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2
    Article Snippet: The cDNA encoding the antibody sequence was cloned into a pcDNA3.4 expression vector (Thermo Fisher Scientific, Waltham, MA, USA), and NP106 was expressed by an Expi-CHO cell system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol, followed by purification using protein G (GE Healthcare Chicago, IL, USA).

    Article Title: Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
    Article Snippet: The variable regions coding sequences were cloned into a pcDNA3.4 (Thermo Fisher) expression vector modified to contain coding sequences of a mouse IgKVIII signal sequence and the appropriate constant regions.

    Article Title: Immunogenicity of a spike protein subunit-based COVID-19 vaccine with broad protection against various SARS-CoV-2 variants in animal studies.
    Article Snippet: The DNA was then ligated into pcDNA3.4 expression vector (Thermo Fisher Scientific) using NEBuilder DNA Assembly kit (New England Biolabs).

    Article Title: Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model.
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific) with a polyhistidine tag at the N-terminus.

    Article Title: Immune evasion and chronological decrease in titer of neutralizing antibody against SARS-CoV-2 and its variants of concerns in COVID-19 patients
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a polyhistidine tag at the N-terminus.

    Article Title: Incomplete humoral response including neutralizing antibodies in asymptomatic to mild COVID-19 patients in Japan
    Article Snippet: The extracellular domain of ACE2 (NCBI, NM_021804.3, 1–708 AA) was cloned from cDNA of human PBMCs into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a streptavidin-binding peptide (SBP) tag or FLAG tag at the C-terminus.

    Modification:

    Article Title: A novel monoclonal antibody against human thymic stromal lymphopoietin for the treatment of TSLP-mediated diseases
    Article Snippet: Gene fragments encoding antibody heavy chain (HC) and light chain (LC) were synthesized by Genewiz (South Plainfield, NJ) or IDT (Coralville, IA) and cloned into pcDNA3.4 expression vector (ThermoFisher Scientific, San Jose, CA).

    Article Title: The Effects of Aβ 1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2
    Article Snippet: The cDNA encoding the antibody sequence was cloned into a pcDNA3.4 expression vector (Thermo Fisher Scientific, Waltham, MA, USA), and NP106 was expressed by an Expi-CHO cell system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol, followed by purification using protein G (GE Healthcare Chicago, IL, USA).

    Article Title: Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
    Article Snippet: The variable regions coding sequences were cloned into a pcDNA3.4 (Thermo Fisher) expression vector modified to contain coding sequences of a mouse IgKVIII signal sequence and the appropriate constant regions.

    Article Title: Immunogenicity of a spike protein subunit-based COVID-19 vaccine with broad protection against various SARS-CoV-2 variants in animal studies.
    Article Snippet: The DNA was then ligated into pcDNA3.4 expression vector (Thermo Fisher Scientific) using NEBuilder DNA Assembly kit (New England Biolabs).

    Article Title: Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model.
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific) with a polyhistidine tag at the N-terminus.

    Article Title: Immune evasion and chronological decrease in titer of neutralizing antibody against SARS-CoV-2 and its variants of concerns in COVID-19 patients
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a polyhistidine tag at the N-terminus.

    Article Title: Incomplete humoral response including neutralizing antibodies in asymptomatic to mild COVID-19 patients in Japan
    Article Snippet: The extracellular domain of ACE2 (NCBI, NM_021804.3, 1–708 AA) was cloned from cDNA of human PBMCs into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a streptavidin-binding peptide (SBP) tag or FLAG tag at the C-terminus.

    Sequencing:

    Article Title: A novel monoclonal antibody against human thymic stromal lymphopoietin for the treatment of TSLP-mediated diseases
    Article Snippet: Gene fragments encoding antibody heavy chain (HC) and light chain (LC) were synthesized by Genewiz (South Plainfield, NJ) or IDT (Coralville, IA) and cloned into pcDNA3.4 expression vector (ThermoFisher Scientific, San Jose, CA).

    Article Title: The Effects of Aβ 1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2
    Article Snippet: The cDNA encoding the antibody sequence was cloned into a pcDNA3.4 expression vector (Thermo Fisher Scientific, Waltham, MA, USA), and NP106 was expressed by an Expi-CHO cell system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol, followed by purification using protein G (GE Healthcare Chicago, IL, USA).

    Article Title: Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
    Article Snippet: The variable regions coding sequences were cloned into a pcDNA3.4 (Thermo Fisher) expression vector modified to contain coding sequences of a mouse IgKVIII signal sequence and the appropriate constant regions.

    Article Title: Immunogenicity of a spike protein subunit-based COVID-19 vaccine with broad protection against various SARS-CoV-2 variants in animal studies.
    Article Snippet: The DNA was then ligated into pcDNA3.4 expression vector (Thermo Fisher Scientific) using NEBuilder DNA Assembly kit (New England Biolabs).

    Article Title: Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model.
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific) with a polyhistidine tag at the N-terminus.

    Article Title: Immune evasion and chronological decrease in titer of neutralizing antibody against SARS-CoV-2 and its variants of concerns in COVID-19 patients
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a polyhistidine tag at the N-terminus.

    Article Title: Incomplete humoral response including neutralizing antibodies in asymptomatic to mild COVID-19 patients in Japan
    Article Snippet: The extracellular domain of ACE2 (NCBI, NM_021804.3, 1–708 AA) was cloned from cDNA of human PBMCs into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a streptavidin-binding peptide (SBP) tag or FLAG tag at the C-terminus.

    Purification:

    Article Title: A novel monoclonal antibody against human thymic stromal lymphopoietin for the treatment of TSLP-mediated diseases
    Article Snippet: Gene fragments encoding antibody heavy chain (HC) and light chain (LC) were synthesized by Genewiz (South Plainfield, NJ) or IDT (Coralville, IA) and cloned into pcDNA3.4 expression vector (ThermoFisher Scientific, San Jose, CA).

    Article Title: The Effects of Aβ 1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2
    Article Snippet: The cDNA encoding the antibody sequence was cloned into a pcDNA3.4 expression vector (Thermo Fisher Scientific, Waltham, MA, USA), and NP106 was expressed by an Expi-CHO cell system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol, followed by purification using protein G (GE Healthcare Chicago, IL, USA).

    Article Title: Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
    Article Snippet: The variable regions coding sequences were cloned into a pcDNA3.4 (Thermo Fisher) expression vector modified to contain coding sequences of a mouse IgKVIII signal sequence and the appropriate constant regions.

    Article Title: Immunogenicity of a spike protein subunit-based COVID-19 vaccine with broad protection against various SARS-CoV-2 variants in animal studies.
    Article Snippet: The DNA was then ligated into pcDNA3.4 expression vector (Thermo Fisher Scientific) using NEBuilder DNA Assembly kit (New England Biolabs).

    Article Title: Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model.
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific) with a polyhistidine tag at the N-terminus.

    Article Title: Immune evasion and chronological decrease in titer of neutralizing antibody against SARS-CoV-2 and its variants of concerns in COVID-19 patients
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a polyhistidine tag at the N-terminus.

    Article Title: Incomplete humoral response including neutralizing antibodies in asymptomatic to mild COVID-19 patients in Japan
    Article Snippet: The extracellular domain of ACE2 (NCBI, NM_021804.3, 1–708 AA) was cloned from cDNA of human PBMCs into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a streptavidin-binding peptide (SBP) tag or FLAG tag at the C-terminus.

    FLAG-tag:

    Article Title: A novel monoclonal antibody against human thymic stromal lymphopoietin for the treatment of TSLP-mediated diseases
    Article Snippet: Gene fragments encoding antibody heavy chain (HC) and light chain (LC) were synthesized by Genewiz (South Plainfield, NJ) or IDT (Coralville, IA) and cloned into pcDNA3.4 expression vector (ThermoFisher Scientific, San Jose, CA).

    Article Title: The Effects of Aβ 1-42 Binding to the SARS-CoV-2 Spike Protein S1 Subunit and Angiotensin-Converting Enzyme 2
    Article Snippet: The cDNA encoding the antibody sequence was cloned into a pcDNA3.4 expression vector (Thermo Fisher Scientific, Waltham, MA, USA), and NP106 was expressed by an Expi-CHO cell system (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol, followed by purification using protein G (GE Healthcare Chicago, IL, USA).

    Article Title: Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
    Article Snippet: The variable regions coding sequences were cloned into a pcDNA3.4 (Thermo Fisher) expression vector modified to contain coding sequences of a mouse IgKVIII signal sequence and the appropriate constant regions.

    Article Title: Immunogenicity of a spike protein subunit-based COVID-19 vaccine with broad protection against various SARS-CoV-2 variants in animal studies.
    Article Snippet: The DNA was then ligated into pcDNA3.4 expression vector (Thermo Fisher Scientific) using NEBuilder DNA Assembly kit (New England Biolabs).

    Article Title: Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model.
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific) with a polyhistidine tag at the N-terminus.

    Article Title: Immune evasion and chronological decrease in titer of neutralizing antibody against SARS-CoV-2 and its variants of concerns in COVID-19 patients
    Article Snippet: Briefly, the extracellular domain of ACE2 was inserted into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a polyhistidine tag at the N-terminus.

    Article Title: Incomplete humoral response including neutralizing antibodies in asymptomatic to mild COVID-19 patients in Japan
    Article Snippet: The extracellular domain of ACE2 (NCBI, NM_021804.3, 1–708 AA) was cloned from cDNA of human PBMCs into the pcDNA3.4 expression vector (Thermo Fisher Scientific, MA, USA) with a streptavidin-binding peptide (SBP) tag or FLAG tag at the C-terminus.



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